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Giardiasis Diagnosis

Diagnostic Tests - Identification of cysts in stool sample, using trichrome or iron hematoxylin staining (19).
- More than one sample is recommended (at least 3 stool samples with two days between each), since the presence of cysts in the stool can be highly irregular, and cysts may not be present until a week after symptoms appear. (14)
- Trophozoites break up rapidly in the stool, and should not be relied upon to measure an infection (14).
- An Enzyme-Linked Immunosorbent Assay (ELISA) may be used to detect Giardia antigens in the stool, and is commercially available (highly sensitive). (14)
- String Test: a patient swallows a capsule with a string attached, and when it is passed into the small intestine, trophozoites stick to the string. The string is then removed and examined for the trophozoites. (19)
- A duodenal biopsy may also be useful to detect trophozoite presence, although trophozoites may be hard to distinguish in the sample (14).
- Seropositivity tests are not recommended, since they cannot distinguish between current and previous infections (19).
- A CBC (complete blood count) is not helpful, because eosinophilia is not present, and the white blood cell count should be normal. (14)
Notes:
Dr. Nelson Crumfield
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(last edited June 20, 2010)
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